Coding

Part:BBa_M11078:Design

Designed by: Forrest Purser   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-18)


OmpC + Copper-Binding Protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 388
    Illegal SpeI site found at 547
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 388
    Illegal SpeI site found at 547
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 388
    Illegal BglII site found at 96
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 388
    Illegal SpeI site found at 547
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 388
    Illegal SpeI site found at 547
    Illegal AgeI site found at 214
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

note: copper-binding protein was inserted in a Pst1 restriction enzyme site ater it was filled in using DNA Polymerase. Two nucletides were inserted at the end of the copper-binding sequence to avoid throwing off the reading frame.


Source

Omp C is native to E.coli

See Xu Z, Lee SY. (1999). Display of Polyhistidine Peptides on the Escherichia coli Cell Surface by Using Outer Membrane Protein C as an Anchoring Motif. Applied and Environmental Microbiology. 65(11): 5142-5147

References